introducing new laboratory-developed molecular methods in the clinical microbiology laboratories

Introducing New Laboratory-Developed Molecular Methods in the Clinical Microbiology Laboratories

Introducing New Laboratory-Developed Molecular Methods in the Clinical Microbiology Laboratories

Commentary The verification of laboratory-developed tests aims to characterize and compare the diagnostic accuracy of a new method to that of a reference one accepted by the laboratory community as the standard of care for a particular analyte or disease. The article entitled " Evaluation of a PCR assay to detect Enterococ-cus faecalis in blood and determine glycopeptides resistance genes: Van ...

Introducing a molecular test into the clinical microbiology laboratory: development, evaluation, and validation.

CONTEXT In the mid-1980s, the polymerase chain reaction methodology for the amplification of minute amounts of target DNA was successfully developed and then introduced into clinical use; such technology has led to a revolution in diagnostic testing. Despite enormous advances in the detection of infectious agents by amplification methods, there are also limitations that must be addressed. OBJ...

Identification of Burkholderia spp. in the clinical microbiology laboratory: comparison of conventional and molecular methods.

Cystic fibrosis (CF) predisposes patients to bacterial colonization and infection of the lower airways. Several species belonging to the genus Burkholderia are potential CF-related pathogens, but microbiological identification may be complicated. This situation is not in the least due to the poorly defined taxonomic status of these bacteria, and further validation of the available diagnostic as...

The Frequency of the Accidental Contamination with Laboratory Samples in Yazd Clinical Laboratories’ personnel in 2011

Abstract Background and Objective: Laboratory personnel have always accidental exposure to clinical samples, which can cause the transmission of infection. This threat can be prevented and controlled by education for the use of safety instruments. The purpose was to determine the frequency of accidental exposure to laboratory samples among Yazd laboratory personnel in 2011. Material and M...

Automation in the Clinical Microbiology Laboratory

The clinical microbiology laboratory has historically been considered “low-tech,” especially when compared to the clinical chemistry laboratory. However, systems are emerging for the clinical microbiology laboratory with the potential to automate almost all areas of testing, including inoculation of primary culture plates, detection of growth on culture media, identification of microorganisms, ...